mRNA, SPLICING and ncRNAs IN GAMETOGENESIS AND PREIMPLANTATION

​

E O’Callaghana*, P Navarrete-Lopez*, M Štiavnická, JM. Sáncheza, M Maroto, E Pericuesta, R Fernández-González, C O’Meara, B Eivers, MM Kelleher, RD. Evans, XM. Mapel, A Lloret-Villas, H Pausch, M Balastegui-Alarcón, M Avilés, A Sanchez-Rodriguez, ERS. Roldan, M McDonal, DA. Kenny, S Fair, A Gutiérrez-Adán*, and P Lonergan* (2023) Adenylate kinase 9 is essential for sperm function and male fertility in mammals. Proceedings of the National Academy of Sciences (PNAS) 120, 42.  www.pnas.org/doi/10.1073/pnas. 2305712120

​

P Navarrete-López, M Maroto, R Fernández-González, E Pericuesta, M Lombó, P Ramos-Ibeas, A Gutiérrez-Adán (2023) Loss of the importin Kpna2 causes infertility in male mice by disrupting the translocation of testis-specific transcription factors. iScience Available online 16 June 2023, 107134.

​

P Navarrete-López, M Lombó, M Maroto, R Fernández-González, E Pericuesta, P Ramos-Ibeas, MT Parra, A Viera, JÁ Suja, A Gutiérrez-Adán (2023) An ancient testis-specific IQ motif containing H gene regulates specific transcript isoform expression during spermatogenesis. Development 150(7). DOI: 10.1242/dev.201334. https://doi.org/10.1242/dev.201334

 

I Gómez-Redondo, E Pericuesta, P Navarrete-Lopez, P Ramos-Ibeas, B Planells, N Fonseca-Balvís, A Vaquero-Rey, R Fernández-González, R Laguna-Barraza, K Horiuchi, and A Gutiérrez-Adán (2022) Zrsr2 and functional U12-dependent spliceosome are necessary for follicular development. iScience 25(2) https://doi.org/10.1016/j.isci.2022.103860

​

Alves, L.Q.; Ruivo, R.; Valente, R.; Fonseca, M.M.; Machado, A.M.; Plön, S.; Monteiro, N.; García-Parraga, D.; Ruiz-Díaz, S.; Sánchez-Calabuig, M.; A. Gutiérrez-Adan* and F. C. Castro* (2021). A drastic shift in the energetic landscape of toothed whale sperm cells Current Biology 2021, https://doi.org/10.1016/j.cub.2021.05.062.

​

I Gómez-Redondo; P. Navarrete, B Planells, A. Gutierrez-Adan (2021) Role of alternative splicing in sex determination in vertebrates. Sexual Development  .https://doi.org/10.1159/000519218

​

I Gómez-Redondo, P Ramos-Ibeas, E Pericuesta, R Fernández-González, R Laguna-Barraza and A Gutiérrez-Adán (2020) Minor splicing factors Zrsr1 and Zrsr2 are essential for early embryo development and 2-cell-like conversion. Int. J. Mol. Sci. 2020, 21(11), 4115; https://doi.org/10.3390/ijms21114115

​

B Planells, I Gomez-Redondo, E Pericuesta, P Lonergan and A Gutiérrez-Adán (2019) Differential isoform expression and alternative splicing in sex determination in mice. BMC Genomics 20:202.

​

Horiuchi K, Perez-Cerezales S, Papasaikas P, Ramos-Ibeas P, López-Cardona AP, Laguna-Barraza R, Fonseca Balvís N, Pericuesta E, Fernández-González R, Planells B, Viera A, Suja JA, Ross PJ, Alén F, Orio L,Rodriguez de Fonseca F, Pintado B, Valcárcel J, Gutiérrez-Adán A. (2018) Impaired Spermatogenesis, Muscle, and Erythrocyte Function in U12 Intron Splicing-Defective Zrsr1 Mutant Mice. Cell Reports 23,143-155.

​

​EPI-TRANSCRIPTOMIC DURING EARLY EMBRYO DEVELOPMENT

​

I Jali, VK Vanamamalai, P Garg, P Navarrete, A Gutiérrez-Adán, S Sharma (2023) Identification and differential expression of long non-coding RNAs and their association with XIST gene during early embryonic development stages of Bos Taurus. International Journal of Biological Macromolecules (ScienceDirect) 229, 896-908 DOI: 10.1016/j.ijbiomac.2022.12.221

 

I Gómez-Redondo; B Planells; S Canovas; E Ivanova; G Kelsey; A Gutiérrez-Adán (2021) Genome-wide DNA methylation dynamics during epigenetic reprogramming in the porcine germline. Clinical Epigenetics 13:27. DOI: 10.1186/s13148-021-01003-x

 

 

B Planells, I Gómez-Redondo, JM Sánchez, M McDonald, A Cánovas, P Lonergan and A Gutiérrez-Adán (2020) Gene expression profiles of bovine genital ridges during sex determination and early differentiation of the gonads. Biology of Reproduction 1-15 doi:10.1093/biolre/ioz170

​

AP López-Cardona, S Perez-Cerezales, R Fernández-González, R Laguna-Barraza, E Pericuesta, N Agirregoitia, E Agirregoitia and A Gutierrez-Adan (2017) The CB1 cannabinoid receptor drives oocyte maturation and embryo development via PI3K/Akt and MAPK pathways. FASEB J., 31(8): 3372-3382.

 

Bermejo-Álvarez P, Ramos-Ibeas P, Park K-E, Powell AP, Vansandt L, Derek Bickhart, Li Ma, Ramirez MA, Gutiérrez-Adán A, Telugu BP (2015) Tet-mediated imprinting erasure in H19 locus following reprogramming of spermatogonial stem cells to induced pluripotent stem cells. Scientific Reports 5, 13691.

  

Bermejo-Álvarez P, Rizos D, Rath D, Lonergan P, Gutiérrez-Adán A (2010). Sex determines the expression level of one third of the actively expressed genes in bovine blastocysts. Proc Natl Acad Sci U S A. 23;107(8):3394-9.

​

Fernández-González R, Moreira P, Bilbao A, Jiménez A, Pérez-Crespo M, Ramírez MA, Rodríguez de Fonseca F, Pintado B, Gutiérrez-Adán A (2004). Long-Term effect of in vitro culture of mouse embryos with serum on mRNA expression of imprinting genes, development and behavior. Proc Natl Acad Sci U S A. 101(16):5880-5.

​

 

SPERM SELECTION AND ART

​

S Ruiz-Diaz; Rosane Mazzarella; P Navarrete-López; R Fernández-González; C de Frutos; M Maroto; C Cucala; P Beltrán-Breña; M Lombó; D Rizos; A Gutiérrez-Adán (2023) Bull spermatozoa selected by thermotaxis exhibit high DNA integrity, specific head morphometry, and improve ICSI outcome. Journal of Animal Science and Biotechnology  (Springer)14(1)11. DOI: 10.1186/s40104-022-00810-3.

 

Lamas-Toranzo I, Hamze JG, Fernández-Fuertes B, Pérez-Cerezales S, Laguna-Barraza R, Fernández-González R, Lonergan P, Gutiérrez-Adán A, Jiménez-Movilla M, Bermejo-Álvarez P (2020) TMEM95 is a sperm membrane protein essential for mammalian fertilization. eLife 2020;9:e53913 doi: 10.7554/eLife.53913

​

R Fernández-González, R Laguna, P Ramos-Ibeas, E Pericuesta, V Alcalde-Lopez, S Perez-Cerezales, A Gutierrez-Adan (2019) Successful ICSI in mice using caput epididymal spermatozoa. Frontiers Cell and Developmental Biology DOI:10.3389/fcell.2019.00346

​

Pérez-Cerezales S, Laguna-Barraza R, Chacón de Castro A, Sánchez-Calabuig MJ, Cano-Oliva E, Castro-Pita FJ, Montoro-Buils L, Pericuesta E, Fernández-González R, Gutiérrez-Adán A. (2018) Sperm selection by thermotaxis improves ICSI outcome in mice. Scientific Reports 8, 2908.

​

Ramos-Ibeas P, Calle A, Fernández-González R, Laguna-Barraza R, Pericuesta E, Calero A, Ramírez MA, Gutiérrez-Adán A (2014). Intracytoplasmic Sperm Injection Using DNA-Fragmented Sperm in Mice Negatively Affects Embryo-Derived Embryonic Stem Cells, Reduces the Fertility of Male Offspring and Induces Heritable Changes in Epialleles. PLoSOne, 9(4):e95625.

​

Fernández-González R, Moreira PN, Pérez-Crespo M, Sánchez-Martín M, Ramírez MA, Pericuesta E, Bilbao A, Bermejo-Álvarez P, de Dios Hourcade J, de Fonseca FR, Gutiérrez-Adán A (2008). Long-term effects of mouse intracytoplasmic sperm injection with DNA-fragmented sperm on health and behavior of adult offspring.  Biol Reprod. 78(4):761-72. 
 

​

​

​

​